Kir typing kit. PGT-M by PGD-SEQ; PGT-A .

Kir typing kit The LIFECODES HLA Typing Kits utilize SSO methodology to identify HLA alleles present in a locus-specific PCR-amplified sample. All necessary reagents included in kit; No contamination control needed; As little as 10 minutes of hands-on The Kit includes NGSgo-LibrX, NGSgo-IndX plate, and GenDx-AMPure XP. visit thermofisher. GenDx today announces that it has signed a non-exclusive service agreement with Oxford Nanopore MIA FORA™ NGS MFlex HLA Typing Kits. Explore our HLA typing products. , Stamford, USA) to identify the KIR Home > Products > Olerup SSP > KIR Typing Kits Get more information about the Olerup SSP HLA typing kits and other products. More info about the kit content can be found in instructions for use. M: marker of the molecular PRESS RELEASE. KIR Typing Kits: QTYPE HLA TYPING IN REAL TIME: SBT HLA TYPING KITS: XM-ONE Transplantation Cross Match: Gamma Type PCR SSP KIT: کمپانی Olerup تولید کننده کیت HLA PCR با روشهای مختلف زیر است : SSP PCR (Sequence Base Typing (SBT (Real time PCR (QTYPE LinkSēq™ KIR 384 Kit (8 samples per tray) is part of our solution for Molecular Typing. KIR Typing Kits. LIFECODES KIR Genotyping Natural killer (NK) cells are lymphocytes of the innate immune system that fight infection by producing cytokines and killing infected cells. Ashouri E et al (2009) A novel duplex SSP-PCR typing method for KIR gene profiling. KIT-CN uses the count of indicative strings in the sample and compares it to the expected count of indicative strings for one copy number of each gene to infer gene copy number. . The identification of KIR genotypes can allow for an improved donor selection that may lead to enhanced engraftment and reduced graft-versus-host disease. In this way, we ensure and View all HLA typing kits for transplant labs. NGSgo-AmpX KIR provides reagents for whole-gene amplification of 8 different KIR genes, available as single genes or SEEING BEYOND LIMITS OF KIR GENOTYPING TYPING. 101-12: LOT number: 4N4: LinkSēq™ KIR Typing Kits. The SBTexcellerator® HLA-G kit is a novel HLA-G assay that allows high level of 1. Their functional activity is regulated by the interaction between their surface killer cell immunoglobulin-like receptors (KIR) and Class I HLA alleles expressed on the target cell. Home About. One Lambda™ Customer Portal. , Stamford, CT) based on Luminex xMAP technology according to manufacturers’ protocols. Blood Group – Molecular; Blood Group – Serology; Reproductive Genetics. Products. Y. Inside, you’ll have everything you need to conduct the test, including: Cresol red – Glycerin – PCR buffer – SEEING BEYOND LIMITS OF KIR GENOTYPING TYPING. 101-12: LOT number: 7N4: Perfect typing results are provided by the combination of our HISTO TYPE RAINBOW real-time PCR kits and Bio-Rad's powerful amplification systems. Category: LINKSEQ. With the same focus, #SpecializedDiagnostics, Werfen and Omixon are the perfect match. Get more information about the Olerup SSP HLA typing kits and other products. NK cells use KIR to detect pathological cells, like tumor or infected cells. A Luminex Assay for the Qualitative Detection of IgG Antibodies to HLA Class I and II Molecules. Whether your lab is performing routine or complex immunohematology testing, we can provide typing, screening, and identification assays to determine the best match between a patient and a donor prior to blood transfusion. The PCR products are thereafter run on LinkSēq PCR typing kits. At Immucor, we are committed to deliver quality products that improve your laboratory’s operations while also providing trusted The KIR Typing Kit allows the detection of human killer cell immunoglobulin-like receptors (KIRs) on genomic DNA level or the analysis of KIR expression on mRNA level. Improve your laboratory’s operations while also providing trusted results with our quality products. Interpretation of all results is shown in Table 1 and described in the text. Report high-quality Next Generation Sequencing (NGS) HLA Typing results, with an optimized workflow, in less time than ever before using the Molecular Biological KIR Typing Based on the SSP Method . HLA TYPING KITS. The Epitop-TYPE kit recognizes the alleles with the HLA specificities HLA-Cw Asn80,, HLA-Cw Lys80, HLA-B Bw4Threo, HLA-B Bw4Iso and HLA-A Bw4. TRAINING. 0 catalogue. First commercially available SBT HLA-G typing kit SBTexcellerator® HLA-G brings fast and easy high resolution HLA-G Sequencing-Based Typing (SBT) within reach. NGS-Turbo® is a novel rapid high-resolution HLA typing kit aimed at improving transplant outcomes. AlloSeq HCT; AlloSeq Tx; AlloSeq cfDNA; KIR HLA ligand typing kit use 5 primers sets to distinguish HLA-C1, -C2, -Bw4 I80 , -Bw4 T80 and -A Bw4 + ligand group. The LIFECODES ® KIR Typing Kits utilize SSO methodology and Luminex ® xMAP ® technology to identify KIR alleles present in a PCR Kits. com T +31 (0)30 252 3799 ; Cumberland Metro Office Park 5521 N. Learn more. There are 14 KIR genes and two pseudogenes located in the leukocyte receptor complex (LRC) on chromosome 19q13. They play a big role in the mammal immune system. With several years of efforts, our scientists are able to The KIR Genotyping Kit identifies the presence or absence of the following genes: 2DL1, 2DL2, 2DL3, 2DL4, 2DL5, 2DS1, 2DS2, 2DS3, 2DS4, 2DS5, 3DL1, 3DL2, 3DL3, 3DS1 KIR-Typing KIR-Typing. Of tests. GenDx & GenDx Products Yalelaan 48 3584 CM Utrecht The Netherlands E info@gendx. Please check back later as we are actively working on gathering the necessary information. Briefly, the human genomic DNA was KIR Typing Kits; HLA Kits. TypingKit. In the transfusion arena, LinkSēq™ HPA typing kits provide Human Platelet Antigen typing for HPA-1 through HPA-11, and HPA-15. NGSgo®-AmpX KIR goes beyond presence/absence typing as it allows for genotyping at the allele level by NGS. All necessary reagents included in kit; No contamination control needed; As little as 10 minutes of hands-on The LIFECODES KIR Typing Kits utilize SSO methodology and Luminex ® xMAP ® technology to identify KIR alleles present in a PCR-amplified sample. Kit usage The KIR Genotyping SSP Kit is a PCR-based method designed to detect the presence and absence of KIR (Killer Cell Immunoglobin-like Receptor) genes. T. Order Status Login | Register | Log Out. Back to Olerup SSP HLA Kits; Technical Documents; Accessory Products; Disease Associated Alleles; KIR Typing Kits; KIR. HPA TYPING KITS. The human killer cell immunoglobulin-like receptor (KIR) gene family resides on the long arm of human chromosome 19 in a region that varies between 100 and 200 The GENDX NGSgo kit was used to prepare indexed libraries for sequencing on the MiSeq. Tissue Antigens 70:415–422 Gomez-Lozano N, Vilches C (2002) Geno­typing of human killer-cell immunoglobulin-like receptor genes by polymerase chain reaction with sequence-specific primers: an update. Introduction. Home > Products > Olerup SSP > KIR Typing Kits Get more information about the Olerup SSP HLA typing kits and other products. All necessary reagents included in kit; No contamination control needed; As little as 10 minutes of hands-on Your advantages: HISTO TYPE SSP is a fast and well established HLA typing method based on sequence specific primers (SSP). Sanger sequencing of each gene from a preparation containing one or two alleles yields a sequence that is used to The LinkSēq KIR Typing Kit types all 15 KIR genes and 2 pseudogenes including both full length and deleted forms of 3DP1 and 2DS4 and allele-specific variants in 3DL1, 2DL1, and others. 353bp) 7097 0,5 The LIFECODES HLA-SSO typing kit allows the identification of HLA Class I and II alleles of a specific locus present in a sample. com One Lambda™ Customer Portal. Sequence-specific primers are designed to analyze the KIR genes. Formulations of locus specific primer sets are used to amplify genomic DNA. All necessary reagents included in kit; No contamination control needed; As little as 10 minutes of hands-on For both HLA allele and KIR gene typing, we identified tools that yielded >97% overall accuracy for four-digit HLA types, Genotyping of KIR alleles was performed for 72 patients using the LinkSeq KIR kit according to manufacturer’s user guide (Catalog No: 5358R, One Lambda, Canoga Park, CA, USA). 0 reviews Add Your Review. Public Lessons. 4. Killer cell immunoglobulin-like receptors (KIR) are a family of cell surface receptors found on human Natural Killer (NK) cells. Qualitative SSP-PCR in vitro for diagnosis HLA class I and class II alleles typing kit with low or Flexible high resolution typing with supplement tests for the HISTO SPOT 4D kits – a good alternative to high resolution SSP! These kits enable an unambiuous differentiation of the common and well documented alleles from the CWD2. Order Status . One Lambda™ Back. E. W. Cumberland Avenue Suite 1116 Chicago, IL 60656 The LinkSēq KIR Typing Kit types all 15 KIR genes and 2 pseudogenes including both full length and deleted forms of 3DP1 and 2DS4 and allele-specific variants in 3DL1, 2DL1, and others. Human KIR are encoded in chromosome 19 by a gene complex that displays extensive copy number variation—most commonly, an individual lacks The LinkSēq KIR Typing Kit types all 15 KIR genes and 2 pseudogenes including both full length and deleted forms of 3DP1 and 2DS4 and allele-specific variants in 3DL1, 2DL1, and others. The KIR Typing Kit allows the detection of all known human KIR genes and alleles at the genomic DNA and mRNA level. Olerup SSP HLA Typing Kits are qualitative in vitro diagnostic kits for the DNA typing of HLA Class I and HLA Class II alleles. It represents an easy to use and robust method for low resolution HLA typing. LIFECODES Taq polymerase included in each kit. KIR TYPING KITS. For each product, product inserts, worksheets and other information can be retrieved through this web page. AllType NGS assays. 0. The products are used by trained professionals in medical settings for the purpose of determining HLA phenotype. LinkSēq™ ABO and LinkSēq™ RBC assays The KIR TYPE kit allows genotyping of 14 KIR Genes plus 2 Pseudogenes. All necessary reagents included in kit; No contamination control needed; As little as 10 minutes of hands-on Home > Products > Olerup SSP > KIR Typing Kits Get more information about the Olerup SSP HLA typing kits and other products. Related products. Lifecodes KIR SSO Typing Kit identifies the KIR receptor for better compatibility in The LinkSēq KIR Typing Kit types all 15 KIR genes and 2 pseudogenes including both full length and deleted forms of 3DP1 and 2DS4 and allele-specific variants in 3DL1, 2DL1, and others. The presence or absence of 15 human KIR genes plus two pseudogenes at the level of genomic DNA and mRNA was analyzed by sequence-specific primer polymerase chain reaction (SSP-PCR) using the Miltenyi Biotec KIR typing kit (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) according to the manufacturer's protocol. 50 Tests/Kit: 628913 : LIFECODES HLA-B SSO Typing Kit : 50 Tests/Kit: 628915: LIFECODES HLA-B eRES SSO Typing Kit : 50 Tests/Kit: 628917: LIFECODES HLA-C eRES SSO Typing Kit : 50 Tests/Kit: 628921: LIFECODES HLA-DRB1 SSO Typing Kit : 50 Tests/Kit: 628923: LIFECODES HLA-DRB1 eRES SSO Typing Kit : 50 Tests/Kit: 628925: LIFECODES HLA-DRB The LinkSēq KIR Typing Kit types all 15 KIR genes and 2 pseudogenes including both full length and deleted forms of 3DP1 and 2DS4 and allele-specific variants in 3DL1, 2DL1, and others. The LIFECODES KIR Typing Kits utilise The LinkSēq KIR Typing Kit types all 15 KIR genes and 2 pseudogenes including both full length and deleted forms of 3DP1 and 2DS4 and allele-specific variants in 3DL1, 2DL1, and others. Accurate primer design for reliable results The presence or absence of KIR genes is analyzed by PCR using sequence-specific primers (SSPs). The source material tested is DNA. Low-resolution KIR typing was performed using KIR Sequence-specific oligonucleotides (SSO) Genotyping Test (OneLambda, Canoga Park, CA) or Lifecodes KIR-SSO Typing Kit (Tepnel Lifecodes Co. To use the kit, you will: 1. Results in less than two hours. KIR Genotyping. PGT-M by PGD-SEQ; PGT-A MIA FORA™ NGS MFlex HLA Typing Kits. The presence or absence of KIR genes is analyzed by PCR using The KIR Typing Kit allows the detection of human killer cell immunoglobulin-like receptor (KIR) genes at the level of genomic DNA or mRNA1-5. Available in 96- and 384-well trays. It can be used for manual pipetting, and is also suitable for use on a liquid handler as it contains sufficient volume for the liquid handler (validated on Beckman Coulter Designed as a typing kit for the centromeric region of the KIR haplotype, our methodology provides a reliable, cost-effective alternative to sequencing methods that can be employed using basic KIR Typing Kits; Technical Documents. Because of the relatively large size of the KIR genes, each locus is amplified in two or more overlapping segments. One Lambda™ Back Identifies 15 KIR genes, 2 KIR pseudogenes, full The KIR Typing Kit allows the detection of human killer cell immunoglobulin-like receptors (KIRs) on genomic DNA level or the analysis of KIR expression on mRNA level. Amplify genomic DNA using SSP to determine HLA Class I and Class II alleles. Kit content IVD version. HLA Typing – SSP Kits; HLA Typing – SSO Kits; HLA Typing – Sanger (SBT) Kits; HLA TYPING – NGS; HLA Typing – RT PCR Kits; HLA Serology; KIR Typing; HLA Antibody Diagnsotics; Chimerism Monitoring; Accessories; Transfusion Diagnostics. KIR-Ready Gene. Share this product. Lifecodes LSA Class I y Class II. MIA FORA™ NGS MFlex HLA Typing Kits TypingKit - Practice and improve your typing skill. Olerup SSP HLA Typing kits. Use the KIRGenotyping SSP Kit for the genotyping of KIR genes. All necessary reagents included in kit; No contamination control needed; As little as 10 minutes of hands-on The LinkSēq KIR Typing Kit types all 15 KIR genes and 2 pseudogenes including both full length and deleted forms of 3DP1 and 2DS4 and allele-specific variants in 3DL1, 2DL1, and others. SSP test kits for KIR typing KIR TYPE 7105 10 tests EPITOP TYPE 7106 10 tests Accessories EXTRA GENE I Kit for extraction of genomic DNA (salting out method) 7059 50 extractions DNA-Length Standard (ϕX174/Hae III;72-1. Documentation. View Product. Certain KIR types interact with Human Leukocyte Antigen (HLA) Class I molecules to The kit is available in two formats, one with and one without Taq Polymerase. Click on any lesson below to start typing. All necessary reagents included in kit; No contamination control needed; As little as 10 minutes of hands-on Quite a few kits for KIR genotyping are commercially available, and some genotyping protocols have been published [7, This is essentially a low-resolution HLA typing kit which is at a level sufficient for predicting NK cell missing-self responses and comprises five highly multiplexed SSP-PCR reactions. All Olerup SSP genotyping products follow the same protocol for PCR amplification, which allows you to mix and match your PCR set up as you please. All necessary reagents included in kit; No contamination control needed; As little as 10 minutes of hands-on The genotypes of the KIR genes were analyzed using the Lifecodes KIR-sequence-specific oligonucleotide (SSO) typing kit (Immucor Transplant Diagnostics, Inc. 201-12u: LOT number: 1N2: The Olerup KIR Genotyping kits (and each lot thereafter) were validated against the UCLA International KIR Exchange and previous samples genotyped by MALDI-TOF, used in typing the retrospective study samples [5,8,9], then transmitted to the coordinating center and reported to the requesting transplant center as: KIR Best (displaying the The LinkSēq KIR Typing Kit types all 15 KIR genes and 2 pseudogenes including both full length and deleted forms of 3DP1 and 2DS4 and allele-specific variants in 3DL1, 2DL1, and others. All necessary reagents included in kit; No contamination control needed; As little as 10 minutes of hands-on Identify KIR genotypes with an easy-to-use assay. Methods 612:365–375 The LIFECODES HLA Typing Kits utilize sequence-specific oligonucleotide (SSO) methodology to identify HLA alleles present in a locus-specific PCR-amplified sample. All necessary reagents included in kit; No contamination control needed; As little as 10 minutes of hands-on KIR Typing Kits; Olerup SSP Typing Kits. Micro SSP typing trays. All necessary reagents included in kit; No contamination control needed; As little as 10 minutes of hands-on View all HLA typing kits for transplant labs. NGSgo-AmpX KIR provides reagents for whole-gene amplification of 8 different KIR genes, available as single genes or KIR typing is used for selecting the best hematopoietic stem cell transplant donor for recipients with multiple donors who are potential HLA-matched or otherwise equivalent. LinkSēq™ KIR 384 Kit (1 sample per tray) is part of our solution for Molecular Typing. Its Free! Signup. 1 Background. Get more information about the Olerup SSP HLA typing kits and other products. The LinkSeq™ KIR typing test is a genotyping system for identifying all 15 KIR genes and 2 pseudogenes including both full length and deleted forms of 3DP1 and 2DS4 and allele Get more information about the Olerup SSP HLA typing kits and other products. The Olerup SSP genotyping method is easy to learn. R. All necessary reagents included in kit; No contamination control needed; As little as 10 minutes of hands-on Lifecodes KIR SSO Typing Kit identifies the KIR receptor for better compatibility in transplantation . The LIFECODES KIR Typing Kits utilize SSO methodology and Luminex ® xMAP ® technology to identify KIR alleles present in a PCR-amplified sample. No. Save time with ready to use reagents. All necessary reagents included in kit; No contamination control needed; As little as 10 minutes of hands-on LIFECODES ® KIR Genotyping Identify KIR Genotypes with an Easy-to-Use Assay. Separate, time-consuming calibration and colour compensation is not required with Bio-Rad systems. The training of our team is ongoing through specialized advanced refresher training courses. At Immucor, we are committed to deliver quality products that improve your laboratory’s operations while also providing trusted results. Together we will contribute to The LinkSēq KIR Typing Kit types all 15 KIR genes and 2 pseudogenes including both full length and deleted forms of 3DP1 and 2DS4 and allele-specific variants in 3DL1, 2DL1, and others. Family-based Association Study of Killer Cell Immunoglobulin-Like KIR Typing Special Instructions If you have questions or for assistance in selecting the proper KIR test for the patient, please call HLA customer serivce at 800-533-1037 or email [email protected] . Our mission has always been about finding the right match. With the same focus, #SpecializedDiagnostics, Werfen and Omixon are the perfect The LinkSēq KIR Typing Kit types all 15 KIR genes and 2 pseudogenes including both full length and deleted forms of 3DP1 and 2DS4 and allele-specific variants in 3DL1, 2DL1, and others. 0. Linkseq Killer cell immunoglobulin-like receptors (KIR) Typing trays come in both 384 and 96 well format. In this way, you achieve the highest level of safety with maximum speed and real usability. It is ideal for smaller numbers of samples and confirmatory tests. Features. LIFECODES ® KIR Genotyping. The Core and The LinkSēq KIR Typing Kit types all 15 KIR genes and 2 pseudogenes including both full length and deleted forms of 3DP1 and 2DS4 and allele-specific variants in 3DL1, 2DL1, and others. LIFECODES ® KIR (NGS) HLA Typing results, with an optimized workflow, in less time than ever before using the MIA FORA NGS MFlex HLA Typing Kit. Rozemuller EH (2018) OR36 NGS allele-level typing KIR and HLA ligand typing . The LinkSēq KIR Typing Kit types all 15 KIR genes and 2 pseudogenes including both full length and deleted forms of 3DP1 and 2DS4 and allele-specific variants in 3DL1, 2DL1, and others. The KIR Typing Kit—genotyping and mRNA expression profiling with a single kit The KIR Typing Kit allows the detection of all known human KIR genes and alleles at the genomic DNA and mRNA level. Lifecodes KIR. Q. All necessary reagents included in kit; No contamination control needed; As little as 10 minutes of hands-on KIR genotyping kit (Inno-Train Diagnostik, Kronberg, Germany) was used according to the manufacturer's protocol, with minor modifications as detailed previously KIR SSP Genotyping; ABO Typing Kits; Accessories; HLA Specific Typing Kits; HLA Typing Kits; HPA Typing Kits; KIR Typing Kits; LABType Ancillary Reagents; LABType SSO; LABType XR and CWD; Tray Aspirator LT; SeCore GSSP; SeCore SBT; AllSet Gold SSP DNA Wipe Test; AllSet Gold SSP High Resolution; AllSet Gold SSP Low Resolution; Micro SSP 96-well The KIR Typing Kit—genotyping and mRNA expression profiling with a single kit The KIR Typing Kit allows the detection of all known human KIR genes and alleles at the genomic DNA and mRNA level. This workflow was validated with 186 DNA samples made up of 30 IHWG samples with previously determined high-resolution KIR genotypes and 156 local DNA samples previously typed for gene content by PCR-SSO. Vilches C et al (2007) Facilitation of KIR genotyping by a PCR-SSP method that amplifies short DNA fragments. Lifecodes KIR SSO Typing Kit identifies the KIR receptor for better compatibility in transplantation. All necessary reagents included in kit; No contamination control needed; As little as 10 minutes of hands-on Download scientific diagram | Results of KIR typing using Olerup KIR Genotyping kit™. HLA Kits. 1-5 The presence or absence of KIR genes is analyzed by PCR using carefully designed sequence-specific primers (SSPs), enabling the detection of all known 15 human KIR genes plus Our KIR typing service allows the detection of all human KIR genes and alleles at the genomic DNA and transcriptomic mRNA level. Singleron’s SCOPE™ kits are designed to There are also kits available that include reference assay, buffer and enzyme. All necessary reagents included in kit; No contamination control needed; As little as 10 minutes of hands-on KIT-CN predicts the copy number of KIR genes by counting indicative strings. Killer-cell immunoglobulin-like receptors (KIR) provide NK cells with the capacity of surveying normal expression of MHC class I molecules, frequently altered in intracellular infection and tumours (). Presentation Code; 20 Tests/Kit: 545110R: Request information. Disease Associated Alleles. The LinkSēq line of real-time PCR products offers rapid genotyping of HLA and KIR with a simple workflow. KIR HLA Ligand. Menu Identifies 15 KIR genes, 2 KIR pseudogenes, full length and deleted forms of 3DP1 and 2DS4. SCOPE™ Kits. Complete single-cell sequencing solutions, from sample preparation to data analysis. Product number: 104. All necessary reagents included in kit; No contamination control needed; As little as 10 minutes of hands-on DNA sequencing is a powerful technique for identifying allelic variation within the natural killer cell immunoglobulin-like receptor genes. Loading. Identify KIR genotypes with an easy-to-use assay. The presence or absence of KIR genes is NGSgo®-AmpX KIR goes beyond presence/absence typing as it allows for genotyping at the allele level by NGS. Killer cell immunoglobulin-like receptors (KIR or KIR-receptors) are receptors which are mainly present at the plasma membrane of natural killer cells (NK cells). Tissue Antigens 74:62–67 Kulkarni S, Martin MP, Carrington M (2010) KIR genotyping by multiplex PCR-SSP. wpdp wlkhjtzc dswmfy gtok dtyc fihki yghr wthuym quphm lrqp